Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration

Authors

  • Liu Liu Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China https://orcid.org/0000-0003-3560-7585
  • Tongtong Yang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Qiqi Jiang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Jiateng Sun Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Lingfeng Gu Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Sibo Wang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Yafei Li Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Bingrui Chen Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Di Zhao Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Rui Sun Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Qiming Wang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
  • Hao Wang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China https://orcid.org/0000-0002-1053-5303
  • Liansheng Wang Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China https://orcid.org/0000-0001-8538-6560

DOI:

https://doi.org/10.17305/bjbms.2022.7770

Keywords:

Proteomic, transcriptomic, heart regeneration, myocardial infarction

Abstract

Research on the regenerative capacity of the neonatal heart could open new avenues for the treatment of myocardial infarction (MI). However, the mechanism of cardiac regeneration remains unclear. In the present study, we constructed a mouse model of heart regeneration and then performed transcriptomic and proteomic analyses on them. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and Gene Set Enrichment Analysis (GSEA) of differentially expressed genes (DEGs) were conducted. Western blot (WB) and qPCR analyses were used to validate the hub genes expression. As a result, gene expression at the mRNA level and protein level is not the same. We identified 3186 DEGs and 42 differentially expressed proteins (DEPs). Through functional analysis of DEGs and DEPs, we speculate that biological processes such as ubiquitination, cell cycle, and oxygen metabolism are involved in heart regeneration. Integrated transcriptomic and proteomic analysis identified 19 hub genes and Ankrd1, Gpx3, and Trim72 were screened out as potential regulators of cardiac regeneration through further expression verification. In conclusion, we combined transcriptomic and proteomic analyses to characterize the molecular features during heart regeneration in neonatal mice. Finally, Ankrd1, Gpx3, and Trim72 were identified as potential targets for heart regeneration therapy.

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Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration

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Published

2022-08-23

How to Cite

1.
Liu L, Yang T, Jiang Q, Sun J, Gu L, Wang S, Li Y, Chen B, Zhao D, Sun R, Wang Q, Wang H, Wang L. Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration. Bosn J of Basic Med Sci [Internet]. 2022Aug.23 [cited 2022Dec.6];. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/7770

Issue

Section

Molecular Biology