Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp

Authors

  • Ana Tenyi Department of Dental Diseases and Normal Dental Morphology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia https://orcid.org/0000-0002-0945-475X
  • Lidija Nemeth Department of Dental Diseases and Normal Dental Morphology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia https://orcid.org/0000-0002-9496-7567
  • Aljaž Golež Institute of Physiology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia https://orcid.org/0000-0003-0552-1465
  • Ksenija Cankar Institute of Physiology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia https://orcid.org/0000-0001-5249-7694
  • Aleksandra Milutinović Institute of histology and embryology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia; International Center for Cardiovascular Diseases MC Medicor Slovenia, Ljubljana, Slovenia https://orcid.org/0000-0003-4142-4814

DOI:

https://doi.org/10.17305/bjbms.2021.6841

Keywords:

Pulse oximetry, electrical sensibility test, histology, volume density of blood vessels and myelinated nerve fibers

Abstract

In dentistry, indirect diagnostic methods such as electrical sensibility testing and pulse oximetry are used to assess the status of the pulp. Our study aimed to determine the correlation between hemoglobin oxygen saturation and vascular volume density (Vvasc). We also wanted to examine an electrical sensibility test and the volume density of myelinated nerve fibers (Vnerv). Twenty-six intact permanent premolars were included in the study. For histological analysis, the pulp tissue was stained with hematoxylin-eosin and immunohistochemically for von Willebrand factor and S100 to detect blood vessels and myelinated nerve fibers, respectively. The stereological analysis was used to determine the Vvasc and Vnerv. Statistical analysis was done using the Pearson correlation test and Welch’s ANOVA test. Histological analysis showed that the pulp tissue was strongly vascularized and innervated. A significant positive correlation was found between Vvasc and hemoglobin oxygen saturation levels (p=0.030). A significant negative correlation was found between Vnerv and the lowest electrical voltage that patient felt (p=0.033). According to the maturity of the dental apex, teeth were divided into a group with open (N=6, OA group) and closed apex (N=20, CA group). We found that pulps in the CA group had higher Vnerv than the OA group (p=0.037). In contrast, there were no significant differences in Vvasc of the pulp tissue (p=0.059), oxygen saturation (p=0.907), or electrical voltage (p=0.113) between both groups. We can conclude that the measurement of pulse oximetry and electrical sensibility test reflect the morphology of healthy pulp tissue independently of the maturity of the dental apex.

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Author Biographies

  • Ana Tenyi, Department of Dental Diseases and Normal Dental Morphology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia

    assistant

  • Lidija Nemeth, Department of Dental Diseases and Normal Dental Morphology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia

    assistant, PhD

  • Aljaž Golež, Institute of Physiology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia

    assistant

  • Ksenija Cankar, Institute of Physiology, Medical Faculty University of Ljubljana, Ljubljana, Slovenia

    prof.PhD

Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp

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Published

01-06-2022

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Section

Pathology

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How to Cite

1.
Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp. Biomol Biomed [Internet]. 2022 Jun. 1 [cited 2024 Jul. 19];22(3):374-81. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/6841

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