The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

Authors

  • Ana Ristic Petrovic Center for Pathology, University Clinical Center Niš, Niš, Serbia; Department of Pathology, Faculty of Medicine, University of Niš, Niš, Serbia https://orcid.org/0000-0002-3359-9828
  • Dragana Stokanović Department of Pharmacology, Faculty of Medicine, University of Niš, Niš, Serbia
  • Slavica Stojnev Center for Pathology, University Clinical Center Niš, Niš, Serbia; Department of Pathology, Faculty of Medicine, University of Niš, Niš, Serbia
  • Milena Potić Floranović Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, Niš, Serbia
  • Miljan Krstić Center for Pathology, University Clinical Center Niš, Niš, Serbia; Department of Pathology, Faculty of Medicine, University of Niš, Niš, Serbia
  • Ivana Djordjević Center for Pathology, University Clinical Center Niš, Niš, Serbia
  • Aleksandar Skakić Clinic for Urology, University Clinical Center Nis, Niš, Serbia; Department of Surgery, Faculty of Medicine, University of Niš, Niš, Serbia
  • Ljubinka Janković Veličković Center for Pathology, University Clinical Center Niš, Niš, Serbia; Department of Pathology, Faculty of Medicine, University of Niš, Niš, Serbia

DOI:

https://doi.org/10.17305/bjbms.2021.6767

Keywords:

bladder cancer, clinical outcome, NOTCH pathway, NOTCH3, immunohistochemistry

Abstract

Disrupted NOTCH activity is a driving event in urothelial bladder cancer (UBC). After activation by hypoxia, the NOTCH3 receptor participates in tumor cell proliferation, acquisition of the epithelial-mesenchymal transition phenotype, and angiogenesis. The aim was to analyze the association of NOTCH3 expression with histopathological and clinical parameters, and to determine its predictive impact on the clinical outcome in UBC patients. The present research included 614 UBC samples incorporated in paraffin tissue microarrays, evaluated by immunohistochemistry for NOTCH3 expression. The accrual period was four years, while the follow-up period was two years. The membranous expression was semi-quantified (0-3), and the mean degree was 1.81±0.94. Criteria for semi-quantification the NOTCH3 expression were the intensity of the staining and the percentage of positive cells. The samples with negative (0) and weak (1) NOTCH3 immunohistochemical (IHC) score were considered negative, while the samples that showed moderate (2) and strong (3) expression were considered positive. Higher degree of positivity was associated with higher risk of cancer-specific mortality (p<0.001). Independent predictors for cancer-specific mortality were NOTCH3 expression and high stage (p<0.001). NOTCH3 expression was not a statistically significant predictor of recurrence-free survival (p=0.816). This study indicated that NOTCH3 is a predictor of poor outcome, suggesting that the NOTCH3 could be potentially reliable IHC marker for selecting the UBC patients that would require more intensive follow-up, especially if they diagnosed in higher stage, with divergent differentiation in pathological report, and without recurrences which would lead them to more frequent medical assessments.

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Association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

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Published

2022-01-24

How to Cite

1.
Ristic Petrovic A, Stokanović D, Stojnev S, Potić Floranović M, Krstić M, Djordjević I, Skakić A, Janković Veličković L. The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients. Bosn J of Basic Med Sci [Internet]. 2022Jan.24 [cited 2022Jul.4];. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/6767

Issue

Section

Pathology