Resveratrol ameliorates neuronal apoptosis and cognitive impairment by activating the SIRT1/RhoA pathway in rats after anesthesia with sevoflurane

Authors

  • Qiaoyun Zhou Department of Anesthesiology, Ningbo Eye Hospital, Ningbo, Zhejiang, China.
  • Yingfeng Deng Department of Anesthesiology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China. https://orcid.org/0000-0002-5511-0835
  • Xuelian Hu Department of Anesthesiology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.
  • Yinye Xu Department of Anesthesiology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China. https://orcid.org/0000-0002-1016-4741

DOI:

https://doi.org/10.17305/bjbms.2021.5997

Keywords:

Resveratrol, neuronal apoptosis, sevoflurane anesthesia, SIRT1/RhoA pathway, postoperative cognitive dysfunction

Abstract

Studies have shown that long-term exposure to sevoflurane (SEV) may cause postoperative cognitive dysfunction. This study aimed to investigate the effects of resveratrol (RES) treatment on the changes in the cognitive function of rats after prolonged anesthesia with SEV. Seventy-six adult male rats were used in this study. The SEV model was established under continuous anesthesia for 6 h. Rats were randomly classified into four groups as follows: control, SEV+vehicle, SEV+pre-RES (RES was administered 24 h before establishing the SEV model), and SEV+post-RES (RES was administered 1 h after establishing the SEV model) groups. Neurobehavioral outcomes and the potential mechanism underlying RES-mediated neuroprotection through the SIRT1/RhoA signaling pathway were evaluated. The water maze test showed that long-term exposure to SEV may lead to loss of learning and memory ability in rats (p<0.05). Compared with the SEV+vehicle group, the RES treatment groups showed significantly improved neurobehavioral scores (p<0.05). Additionally, the SEV+pre-RES group had a better outcome than the SEV+vehicle group on days 1 or 2 (p<0.05), unlike the SEV+post-RES group (p>0.05). Western blotting showed that SIRT1, RhoA, and cleaved Caspase-3 (CC3) expression significantly increased in the SEV+vehicle group (p<0.05), while Bcl2 expression decreased (p < 0.05). RES treatment further upregulated SIRT1 and Bcl2 expression and downregulated the expression of RhoA and CC3 (p<0.05). In conclusion, RES treatment improved cognitive dysfunction by reducing neuronal apoptosis in adult rats exposed to SEV. RES partly exerted a neuroprotective effect through the activation of the SIRT1/RhoA signaling pathway.

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Author Biographies

  • Qiaoyun Zhou, Department of Anesthesiology, Ningbo Eye Hospital, Ningbo, Zhejiang, China.

     

  • Xuelian Hu, Department of Anesthesiology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.

     

  • Yinye Xu, Department of Anesthesiology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.

     

Resveratrol ameliorates neuronal apoptosis and cognitive impairment by activating the SIRT1/RhoA pathway in rats after anesthesia with sevoflurane

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Published

01-02-2022

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Section

Pharmacology

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How to Cite

1.
Resveratrol ameliorates neuronal apoptosis and cognitive impairment by activating the SIRT1/RhoA pathway in rats after anesthesia with sevoflurane. Biomol Biomed [Internet]. 2022 Feb. 1 [cited 2024 Dec. 11];22(1):110-7. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/5997