Impacts of N-Butylphthalide on expression of growth factors in rats with focal cerebral ischemia

Authors

  • Yan Jiang Department of Neurology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China
  • Leyu Sun Department of Neurology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China
  • Xiaoyan Xuan Department of Microbiology and Immunology, Basic Medical College Zhengzhou University, Zhengzhou, Henan, China
  • Jianping Wang Department of Neurology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

DOI:

https://doi.org/10.17305/bjbms.2016.560

Keywords:

N-butylphthalide, rats with cerebral ischemia and reperfusion, vascular endothelial growth factor, transforming growth factor-β1

Abstract

This study investigates the impacts of n-butylphthalide (NBP) on the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) in rats with focal cerebral ischemia. The thread embolization method was used to prepare the rat model of cerebral ischemia-reperfusion (CIR). The animals were divided into a sham operation group, a model control group and NBP treatment group. The NBP group was orally administered 25 mg/kg NBP twice a day after the surgery. The immunohistochemistry and reverse transcription-polymerase chain reaction were performed to observe the protein and mRNA expressions of VEGF and TGF-β 16 hours, 1 day and 2 days after inducing CIR. The mRNA and protein expressions of VEGF and TGF-β1 in the model control group and the NBP treatment group were all increased after CIR, and those of the NBP treatment group at each post-CIR time point were higher than the model control group (p < 0.01). After CIR, the expressions of VEGF and TGF-β1 increased, suggesting that VEGF and TGF-β1 exhibited protective effects towards the ischemic brain injuries, and that NBP could upregulate the expressions of VEGF and TGF-β1 in the peri-infarcted area, thus possibly protecting the ischemic brain tissues through this mechanism.

Author Biography

  • Xiaoyan Xuan, Department of Microbiology and Immunology, Basic Medical College Zhengzhou University, Zhengzhou, Henan, China
    Department of Neurology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

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Impacts of N-Butylphthalide on expression of growth factors in rats with focal cerebral ischemia

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01-01-2016

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1.
Impacts of N-Butylphthalide on expression of growth factors in rats with focal cerebral ischemia. Biomol Biomed [Internet]. 2016 Jan. 1 [cited 2024 Mar. 29];16(2):102-7. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/560