Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases

  • Sebnem Batur Department of Pathology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey https://orcid.org/0000-0001-6577-8970
  • Onur Dulger Department of Pathology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey https://orcid.org/0000-0002-1155-7790
  • Sermin Durak Department of Pathology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey https://orcid.org/0000-0001-6741-4345
  • Perran Fulden Yumuk Department of Internal Medicine, Division of Medical Oncology, Pendik Education and Research Hospital, School of Medicine, Marmara University, Istanbul, Turkey https://orcid.org/0000-0001-8650-299X
  • Hale Basak Caglar Department of Radiation Oncology, Medipol University, Istanbul, Turkey
  • Emine Bozkurtlar Department of Pathology, School of Medicine, Marmara University, Istanbul, Turkey https://orcid.org/0000-0002-1034-9236
  • Suheyla Bozkurt Department of Pathology, School of Medicine, Marmara University, Istanbul, Turkey https://orcid.org/0000-0002-5947-947X
  • Ebru Tastekin Department of Pathology, Medical Faculty, Trakya University, Edirne, Turkey
  • Irfan Cicin Department of Internal Medicine, Division of Medical Oncology, Trakya University, Edirne, Turkey https://orcid.org/0000-0002-7584-3868
  • Rengin Ahiskali Department of Pathology, School of Medicine, Marmara University, Istanbul, Turkey
  • Rashad Rzazade Department of Radiation Oncology, Anatolia Health Center, Gebze, Turkey
  • Asli Cakir Department of Internal Medicine, Division of Medical Oncology, Medipol University, Istanbul, Turkey https://orcid.org/0000-0003-0128-6947
  • Buge Oz Department of Pathology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey https://orcid.org/0000-0003-3201-1313
Keywords: Programmed death-ligand 1, non-small-cell lung cancer, NSCLC, tumor immunology, PD-L1, brain metastasis, CD8 lymphocytes, tumor infiltrating lymphocytes, TILs

Abstract

Programmed death-ligand 1 (PD-L1) is suggested to be a predictive biomarker in non-small-cell lung carcinoma (NSCLC). However, the differential expression of PD-L1 in primary lung tumor vs. synchronous metastases, especially brain metastasis (BM), remains unclear. This study assessed the concordance of PD-L1 expression on tumor cells and tumor-infiltrating lymphocytes (TILs) and CD8+ TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values <1%, 1–49%, and ≥50%. CD3 and CD8 positivity in TILs was evaluated semi-quantitatively and the proportion of CD3+/CD8+ TILs was determined. PD-L1 expression on tumor cells and TILs was evaluated in relation to CD3+/CD8+ TIL proportions and the intensity of CD8+ TILs between the paired primary lung and BM tissues. In the primary lung tumors, PD-L1 positivity was observed in 25%, 37.5%, and 37.5% cases for the cutoff values <1%, 1–49%, and ≥50%, respectively. PD-L1 expression on tumor cells was strongly correlated between the paired primary lung and BM tissues, in all cutoff groups. However, PD-L1 expression on TILs and the proportion of CD3+/CD8+ TILs were not strongly correlated in all three groups between the paired primary lung tumors and BMs. The intensity of CD8+ TILs was concordant in only 54.16% of the paired primary lung tumors and BMs. This study showed a high concordance of PD-L1 expression in neoplastic cells between primary NSCLC and synchronous BMs.

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Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases
Published
2020-08-03
How to Cite
1.
Batur S, Dulger O, Durak S, Yumuk PF, Caglar HB, Bozkurtlar E, Bozkurt S, Tastekin E, Cicin I, Ahiskali R, Rzazade R, Cakir A, Oz B. Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases. Bosn J of Basic Med Sci [Internet]. 2020Aug.3 [cited 2020Aug.8];20(3):329-35. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/4474
Section
Pathology