Teucrium Polium Plant Extract Provokes Substantial Cytotoxicity at the Early Stage of Embryonic Development

  • Shaikha S. Al-Qahdi College of Medicine, Qatar University; Biomedical Research Centre, Qatar University, Doha, Qatar
  • Nada Alzohari College of Medicine, Qatar University; Biomedical Research Centre, Qatar University, Doha, Qatar
  • Abrar Y. Alsaid College of Medicine, Qatar University; Biomedical Research Centre, Qatar University, Doha, Qatar
  • Anas A. Ashour College of Medicine, Qatar University; Biomedical Research Centre, Qatar University, Doha, Qatar
  • Tahar Aboulkassim Segal Cancer Centre, Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada
  • Semir Vranic College of Medicine, Qatar University, Doha, Qatar http://orcid.org/0000-0001-9743-7265
  • Ala-Eddin Al Moustafa College of Medicine, Qatar University; Biomedical Research Centre, Doha, Qatar; McGill University, Montreal, Quebec, Canada; Syrian Research Cancer Centre of the Syrian Society against Cancer, Aleppo, Syria
  • Ashraf A. Khalil College of Pharmacy, Qatar University, Doha, Qatar
Keywords: Teucrium Polium, Medicinal plant, Embryo, Angiogenesis, Toxicity, Gene deregulations

Abstract

The aim of this study is to explore the outcome of Teucrium polium (TP) medicinal plant consumption on the early stage of fetal development. We used the chicken embryo at 3 days of incubation as a model to evaluate the effect of TP plant extract during embryogenesis. In addition, quantitative polymerase chain reaction (qPCR) was applied to explore the expression of six genes related to cell proliferation, apoptosis, sur­vival, angiogenesis, and migration. Our data revealed that TP exposure inhibits angiogenesis of the chicken embryo and its chorioallantoic membrane. In addition, we found that TP extract significantly harms the normal development of the embryos since around 95% of TP-exposed embryos died after 1–3 days of treatment. Macroscopic examination did not show any anomalies in these embryos. However, qPCR analysis of activation transcription factor-3, B-cell lymphoma-2, caspase 8, inhibin subunit beta A, vascular endothelial growth factor-C, and Cadherin-6 type-2 genes revealed that these genes are considerably deregulated in heart and brain tissues from TP-exposed embryos in comparison with their matched tissues from unexposed ones. Our study implies that TP plant can have very toxic effects on the early stage of the embryo. Therefore, it is important to alert expectant women to avoid the use of this medicinal plant during pregnancy.

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Author Biography

Ashraf A. Khalil, College of Pharmacy, Qatar University, Doha, Qatar

Department of Pharmaceutical Sciences

Published
2019-02-12
How to Cite
1.
Al-Qahdi SS, Alzohari N, Alsaid AY, Ashour AA, Aboulkassim T, Vranic S, Al Moustafa A-E, Khalil AA. Teucrium Polium Plant Extract Provokes Substantial Cytotoxicity at the Early Stage of Embryonic Development. Bosn J of Basic Med Sci [Internet]. 2019Feb.12 [cited 2019Oct.19];19(1):67-1. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/4052
Section
Pharmacology