p16/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women with abnormal cytology under 30 years old

  • Laurențiu Pirtea Department of Obstetrics and Gynecology, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Cristina Secosan Department of Obstetrics and Gynecology, County Hospital Timişoara, Timișoara, Romania
  • Madalin Margan Department of Obstetrics and Gynecology, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Lavinia Moleriu Department of Informatics and Statistics, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Oana Balint Department of Obstetrics and Gynecology, County Hospital Timişoara, Timișoara, Romania
  • Dorin Grigoras Department of Obstetrics and Gynecology, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Ioan Sas Department of Obstetrics and Gynecology, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Florin Horhat Department of Microbiology, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Adelina Jianu Department of Anatomy, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
  • Răzvan Ilina Department of Surgery, University of Medicine and Pharmacy “Victor Babeş” Timişoara, Timișoara, Romania
Keywords: Human papillomavirus, immunocytochemistry, p16/Ki-67 dual staining, HPV genotyping

Abstract

Due to a high rate of transient human papillomavirus (HPV) infection, HPV genotyping has a low specificity for high-grade cervical lesions, especially in young women. p16/Ki-67 dual immunocytochemical staining can also be used for the detection of oncogenic changes in cervical cells. Our aim was to compare the performance of p16/Ki-67 dual staining and HPV genotyping in the detection of high-grade cervical lesions in patients with atypical squamous cells of undetermined significance (ASCUS)/low-grade squamous intraepithelial lesion (LSIL) on Pap smear. We retrospectively analyzed 310 patients with ASCUS/LSIL on Pap smear, who underwent colposcopy. Among these, 161 patients with suspected lesions detected by colposcopy were referred for biopsy. HPV genotyping by LINEAR ARRAY HPV Genotyping Test (CE-IVD) and p16/Ki-67 dual staining by CINtec PLUS Cytology kit was performed prior to cervical biopsy. The overall sensitivity and specificity of HPV genotyping for the detection of cervical intraepithelial neoplasia (CIN) 2-3 was 79% and 72%, respectively in patients with ASCUS, and 85% and 64%, respectively in patients with LSIL. For p16/Ki-67 test, sensitivity and specificity rate was 66% and 93%, respectively in ASCUS and 59% and 79%, respectively in LSIL group. The specificity of p16/Ki-67 staining was significantly higher in both groups in patients aged <30 years compared to patients >30 years old (p < 0.001). Our results showed that p16/Ki-67 dual staining has a higher specificity compared to HPV genotyping, especially in patients under 30 years old. This indicates the usefulness of p16/Ki-67 testing in the triage of patients with ASCUS/LSIL and <30 years old, prior to referral for colposcopy and biopsy.

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p16/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women under 30 years old
Published
2018-06-20
How to Cite
1.
Pirtea L, Secosan C, Margan M, Moleriu L, Balint O, Grigoras D, Sas I, Horhat F, Jianu A, Ilina R. p16/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women with abnormal cytology under 30 years old. Bosn J of Basic Med Sci [Internet]. 2018Jun.20 [cited 2019Aug.26];00. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/3560
Section
Pathology