Use of immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex from liquid culture

  • Anika Považan Institute for Pulmonary Diseases of Vojvodina, Center for Microbiology, Immunology and Virusology
  • Anka Vukelić Institute for Pulmonary Diseases of Vojvodina, Center for Microbiology, Immunology and Virusology
  • Tijana Savković Medical school “7th April”
  • Tatjana Kurucin Institute for Pulmonary Diseases of Vojvodina, Center for Microbiology, Immunology and Virusology
Keywords: Mycobacterium tuberculosis complex, identification, immunochromatographic assay

Abstract

A new, simple immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in liquid cultures has been developed. The principle of the assay is binding of the Mycobacterium tuberculosis complex specific antigen to the monoclonal antibody conjugated on the test strip. The aim of this study is evaluation of the performance of immunochromatographic assay in identification of Mycobacterium tuberculosis complex in primary positive liquid cultures of BacT/Alert automated system.

A total of 159 primary positive liquid cultures were tested using the immunochromatographic assay (BD MGIT TBc ID) and the conventional subculture, followed by identification using biochemical tests.

Of 159 positive liquid cultures, using the conventional method, Mycobacterium tuberculos is was identified in 119 (74.8%), nontuberculous mycobacteria were found in 4 (2.5%), 14 (8.8%) cultures were contaminated and 22 (13.8%) cultures were found to be negative. Using the immunochromatographic assay, Mycobacterium tuberculosis complex was detected in 118 (74.2%) liquid cultures, and 41 (25.8%) tests were negative. Sensitivity, specificity, positive and negative predictive values of the test were 98.3%; 97.5%; 99.15%; 95.12%, respectively. The value of kappa test was 0.950, and McNemar test was 1.00.

The immunochromatographic assay is a simple and rapid test which represents a suitable alternative to the conventional subculture method for the primary identification of Mycobacterium tuberculosis complex in liquid cultures of BacT/Alert automated system.

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Use of immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex from liquid culture
Published
2012-02-20
How to Cite
1.
Považan A, Vukelić A, Savković T, Kurucin T. Use of immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex from liquid culture. Bosn J of Basic Med Sci [Internet]. 2012Feb.20 [cited 2020Aug.15];12(1):33-6. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/2531
Section
Microbiology