Genetic polymorphisms of ADH1B, ADH1C and ALDH2 in Turkish alcoholics: lack of association with alcoholism and alcoholic cirrhosis

Authors

  • Sezgin Vatansever Ege University Medical School
  • Fatih Tekin Ege University Medical School
  • Esin Salman Ege University Medical School
  • Ender Altintoprak Ege University Medical School
  • Hakan Coskunol Ege University Medical School
  • Ulus Salih Akarca Ege University Medical School

DOI:

https://doi.org/10.17305/bjbms.2015.242

Keywords:

alcohol, gene polymorphism, alcoholism, cirrhosis, Turkey

Abstract

No data exists regarding the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) gene polymorphisms in Turkish alcoholic cirrhotics. We studied the polymorphisms of ADH1B, ADH1C and ALDH2 genes in alcoholic cirrhotics and compared the results with non-cirrhotic alcoholics and healthy volunteers. Overall, 237 subjects were included for the study: 156 alcoholic patients (78 cirrhotics, 78 non-cirrhotic alcoholics) and 81 healthy volunteers. Three different single-nucleotide-polymorphism genotyping methods were used. ADH1C genotyping was performed using a polymerase chain reaction-restriction fragment length polymorphism method. The identified ADH1C genotypes were named according to the presence or absence of the enzyme restriction sites. ADH1B (Arg47Hys) genotyping was performed using the allele specific primer extension method, and ALDH2 (Glu487Lys) genotyping was performed by a multiplex polymerase chain reaction using two allele-specific primer pairs. For ADH1B, the frequency of allele *1 in the cirrhotics, non-cirrhotic alcoholics and healthy volunteers was 97.4%, 94.9% and 99.4%, respectively. For ADH1C, the frequency of allele *1 in the cirrhotics, non-cirrhotic alcoholics and healthy volunteers was 47%, 36.3% and 45%, respectively. There was no statistical difference between the groups for ADH1B and ADH1C (p>0.05). All alcoholic and non-alcoholic subjects (100%) had the allele *1 for ALDH2. The obtained results for ADH1B, ADH1C, and ALDH gene polymorphisms in the present study are similar to the results of Caucasian studies. ADH1B and ADH1C genetic variations are not related to the development of alcoholism or susceptibility to alcoholic cirrhosis. ALDH2 gene has no genetic variation in the Turkish population.

Author Biographies

  • Sezgin Vatansever, Ege University Medical School
    Department of Gastroenterology
  • Fatih Tekin, Ege University Medical School
    Department of Gastroenterology
  • Esin Salman, Ege University Medical School
    Department of Gastroenterology
  • Ender Altintoprak, Ege University Medical School
    Department of Psychiatry
  • Hakan Coskunol, Ege University Medical School
    Department of Psychiatry
  • Ulus Salih Akarca, Ege University Medical School
    Department of Gastroenterology

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Genetic polymorphisms of ADH1B, ADH1C and ALDH2 in Turkish alcoholics: lack of association with alcoholism and alcoholic cirrhosis

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Published

17-05-2015

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1.
Genetic polymorphisms of ADH1B, ADH1C and ALDH2 in Turkish alcoholics: lack of association with alcoholism and alcoholic cirrhosis. Biomol Biomed [Internet]. 2015 May 17 [cited 2024 Mar. 28];15(2):37-41. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/242