Rat bone marrow stem cells isolation and culture as a bone formative experimental system

  • Amer Smajilagić Clinic for Maxillofacial Surgery, Clinical University Center
  • Mufida Aljičević Institute for Microbiology, Medical Faculty, University of Sarajevo
  • Amira Redžić Institute for Human Genetics and Biology, Medical Faculty, University of Sarajevo
  • Selma Filipović Institute for the Animal Surgery, Veterinary Faculty, University of Sarajevo
  • Alena C. Lagumdžija Capio Diagnostic, Unilabs, Lerbäcksgränd
Keywords: Adult stem cells, bone marrow, bone marrow stem cells

Abstract

Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility. The cells obtained by plastic adherence were cultured and serially passaged in the osteoinductive medium to differentiate into the osteocytes. Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs). Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs as autologue cells model showed high osteogenetic potential and calcification capatibility in vitro. In future should be used as alternative method for bone transplantation in Regenerative Medicine.

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Rat bone marrow stem cells isolation and culture as a bone formative experimental system
Published
2013-02-20
How to Cite
1.
Smajilagić A, Aljičević M, Redžić A, Filipović S, C. Lagumdžija A. Rat bone marrow stem cells isolation and culture as a bone formative experimental system. Bosn J of Basic Med Sci [Internet]. 2013Feb.20 [cited 2020Aug.15];13(1):27-0. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/2409
Section
Molecular Biology