Molecular – genetic variance of RH blood group system within human population of Bosnia and Herzegovina

Lejla Lasić; Naida Lojo-Kadrić; Elma Silajdžić; Lejla Pojskić; Rifat Hadžiselimović; Naris Pojskić

doi:10.17305/bjbms.2013.2403

Authors

Lejla Lasić Institute for Genetic Engineering and Biotechnology Sarajevo
Naida Lojo-Kadrić Institute for Genetic Engineering and Biotechnology Sarajevo
Elma Silajdžić Institute for Genetic Engineering and Biotechnology Sarajevo
Lejla Pojskić Institute for Genetic Engineering and Biotechnology Sarajevo
Rifat Hadžiselimović Institute for Genetic Engineering and Biotechnology Sarajevo
Naris Pojskić Institute for Genetic Engineering and Biotechnology Sarajevo

DOI:

https://doi.org/10.17305/bjbms.2013.2403

Keywords:

RHD gene, RHCE gene, frequency, Rh blood group system, allele specific PCR method

Abstract

There are two major theories for inheritance of Rh blood group system: Fisher - Race theory and Wiener theory. Aim of this study was identifying frequency of RHDCE alleles in Bosnian - Herzegovinian population and introduction of this method in screening for Rh phenotype in B&H since this type of analysis was not used for blood typing in B&H before. Rh blood group was typed by Polymerase Chain Reaction, using the protocols and primers previously established by other authors, then carrying out electrophoresis in 2-3% agarose gel. Percentage of Rh positive individuals in our sample is 84.48%, while the percentage of Rh negative individuals is 15.52%. Inter-rater agreement statistic showed perfect agreement (K=1) between the results of Rh blood system detection based on serological and molecular-genetics methods. In conclusion, molecular - genetic methods are suitable for prenatal genotyping and specific cases while standard serological method is suitable for high-throughput of samples.