Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma

Amirala Aghbali; Sepideh Vosough Hosseini; Abbas r Delaza; Nader Kalbasi Gharavi; Fatemeh Zare Shahneh; Mona Orangi; Ali Bandehagh; Behzad Baradaran

doi:10.17305/bjbms.2013.2360

Authors

Amirala Aghbali Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences
Sepideh Vosough Hosseini Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences
Abbas r Delaza Drug Applied Research Center, Tabriz University of Medical Sciences
Nader Kalbasi Gharavi Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences
Fatemeh Zare Shahneh Immunology Research Center, Tabriz University of Medical Sciences
Mona Orangi Immunology Research Center, Tabriz University of Medical Sciences
Ali Bandehagh Drug Applied Research Center, Tabriz University of Medical Sciences
Behzad Baradaran Drug Applied Research Center, Tabriz University of Medical Sciences

DOI:

https://doi.org/10.17305/bjbms.2013.2360

Keywords:

cytotoxicity, apoptosis, Anticancer, oral squamous cell carcinoma, grape seed extract

Abstract

Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan’s test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma.