Effect of co-administration of morphine and nicotine on cardiovascular function in two-kidney one clip hypertensive (2K1C) rats

  • Motahareh Zeinivand Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences
  • Mohammad Reza Rahman Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences
  • Mohammad Allatavakoli Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences
  • Ali Shamsizadeh Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences
  • Gholamhossin Hassanshahi Molecular Medicine Research Centre, Enq St. RafsanjanUniversity of Medical Sciences
  • Hossien Rezazadeh Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences
  • Ali Asghar Pourshanazari Physiology-Pharmacology Research Center, Enq St. Rafsanjan University of Medical Sciences Department of Physiology, Hezar-Jerib St. Isfahan University of Medical Sciences
Keywords: baroreflex sensitivity, blood pressure, nitric oxide, morphine, nicotine

Abstract

Cardiovascular morbidity and mortality are potentiated with smoking and hypertension. The aim of this study was to investigate the effects of morphine and nicotine co-administration on cardiovascular function in two-kidney one-clip hypertensive (2K1C) rats. Thirty-two male rats were divided into four groups as follow: Vehicle, morphine, nicotine and nicotine + morphine. All drugs were administered for 8 weeks. Baroreflex sensitivity (BRS), heart rate and blood pressure were measured using a Power Lab data acquisition. Plasma rennin activity (PRA) and serum concentration of nitric oxide (NO) were measured using Elisa method. To induce hypertension, the renal artery of left kidney was clipped for 8 weeks. A significant decrease in systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) was observed in nicotine + morphine group compared to vehicle and nicotine groups (p<0.05). Serum concentration of NO was lower in nicotine + morphine group compared to morphine group and significantly higher than nicotine group. The BRS was lower in the nicotine + morphine group compared to other groups. The PRA level was higher in nicotine + morphine compared to morphine group but it was higher than nicotine group. This study demonstrated that prolonged co-consumption of morphine and nicotinedecreased PRA and blood pressure and increased the serum concentration of NO in hypertensive rats. Co-administration of morphine and nicotine decreased BRS in 2kic hypertensive rats probably via central nervous system.

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Effect of co-administration of morphine and nicotine on cardiovascular function in two-kidney one clip hypertensive (2K1C) rats
Published
2013-08-20
How to Cite
1.
Zeinivand M, Reza Rahman M, Allatavakoli M, Shamsizadeh A, Hassanshahi G, Rezazadeh H, Asghar Pourshanazari A. Effect of co-administration of morphine and nicotine on cardiovascular function in two-kidney one clip hypertensive (2K1C) rats. Bosn J of Basic Med Sci [Internet]. 2013Aug.20 [cited 2020Aug.7];13(3):140-5. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/2345
Section
Pharmacology