BIX01294 suppresses osteoclast differentiation on mouse macrophage-like Raw264.7 cells

  • Hiromasa Tsuda Department of Biochemistry, School of Dentistry, Nihon University Division of Functional Morphology and Immunology, Dental Research Center, School of Dentistry, Nihon University
  • Ning Zhao Department of Endodontics, School of Dentistry, Shandong University
  • Kenichi Imai Department of Oral Microbiology, School of Dentistry, Nihon University Division of Pathobiology, Dental Research Center, School of Dentistry, Nihon University
  • Kuniyasu Ochiai Department of Oral Microbiology, School of Dentistry, Nihon University Division of Pathobiology, Dental Research Center, School of Dentistry, Nihon University
  • Pishan Yang Department of Endodontics, School of Dentistry, Shandong University
  • Naoto Suzuki Department of Biochemistry, School of Dentistry, Nihon University Division of Functional Morphology and Immunology, Dental Research Center, School of Dentistry, Nihon University
Keywords: osteoclast differentiation, BIX01294, histone methyltransferase, Raw264.7 cell

Abstract

Gene expressionis controlled by epigenetic mechanisms including histone methylation. Osteoclasts are bone-resorptive cells that differentiate from hematopoietic-precursor cells by receptor activator of nuclear factor-KB ligand (RANKL) stimulation. Although BIX01294, a specific inhibitor of G9a, which works as a histone H3 lysine 9 (H3K9) methyltransferase, reportedly changes cellular differentiational stage, its effect on osteoclast differentiation is unclear. In this study, the effects of BIX01294 on osteoclast differentiation were examined. Here, we showed that BIX01294 dose-dependently reduced RANKL-induced tartrate-resistant acid phosphatase positive multinuclear osteoclast-like cell differentiation from murine macrophage-like Raw264.7 cells. During differentiation, growth rates reduced only less than 14% of those of cells stimulated with RANKL alone by BIX01294 treatment. Moreover, western blot analysis showed that BIX01294 reduced RANKL-induced carbonic anhydrase II and cathepsin K production and decreased RANKL-induced nuclear factor of activated T-cell c1, a master regulatory transcription factor, production during osteoclast differentiation. These results suggest that BIX01294 suppresses RANKL-induced osteoclast differentiation. This is the first report about the effect of BIX01294 on osteoclast differentiation.

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BIX01294 suppresses osteoclast differentiation on mouse macrophage-like Raw264.7 cells
Published
2013-11-20
How to Cite
1.
Tsuda H, Zhao N, Imai K, Ochiai K, Yang P, Suzuki N. BIX01294 suppresses osteoclast differentiation on mouse macrophage-like Raw264.7 cells. Bosn J of Basic Med Sci [Internet]. 2013Nov.20 [cited 2020Aug.4];13(4):271-5. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/2339
Section
Molecular Biology