Angiotensinogen (AGT) gene missense polymorphisms (rs699 and rs4762) and diabetic nephropathy in Caucasians with type 2 diabetes mellitus

Jana Makuc; Maja Šeruga; Matej Završnik; Ines Cilenšek; Daniel Petrovič

doi:10.17305/bjbms.2017.1823

Authors

Jana Makuc Department of Internal Medicine, General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia
Maja Šeruga Department of Internal Medicine, General Hospital Murska Sobota, Murska Sobota, Slovenia
Matej Završnik Department of Endocrinology and Diabetology, Division of Internal Medicine, University Medical Centre Maribor, Maribor, Slovenia
Ines Cilenšek Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Daniel Petrovič Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

DOI:

https://doi.org/10.17305/bjbms.2017.1823

Keywords:

Angiotensinogen, AGT, rs699, rs4762, diabetic nephropathy, T2DM, type 2 diabetes mellitus

Abstract

Gene polymorphisms associated with the renin–angiotensin–aldosterone system (RAAS) have been extensively studied in diabetic nephropathy (DN) patients, due to therapeutic potential of targeting the RAAS and slowing down the disease progression. The aim of our study was to examine the association between angiotensinogen (AGT) gene polymorphisms (rs699 and rs4762) and DN in Caucasians with type 2 diabetes mellitus (T2DM). A total of 651 unrelated Slovenian (Caucasian) T2DM patients were tested for AGT rs699 and rs4762 polymorphisms using a novel fluorescence-based kompetitive allele-specific polymerase chain reaction (KASPar) assay. A study group consisted of 276 T2DM patients with DN, while control group included 375 patients without DN but who have had T2DM for >10 years. For rs699 polymorphism, the frequencies of GG, GA and AA genotypes were 20.6%, 52.2% and 27.2%, respectively in T2DM patients and 23.4%, 48.1% and 28.5%, respectively in controls. The distributions of GG, GA and AA genotypes for rs4762 polymorphism were 73.9%, 23.2% and 2.9%, respectively in T2DM patients and 70.4%, 27.5% and 2.1%, respectively in controls. No significant differences in the allele frequencies were found between T2DM patients and controls for both polymorphisms. AGT rs699 and rs4762 missense polymorphisms are not associated with DN in our subset of Slovenian T2DM patients.

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Author Biographies

Jana Makuc, Department of Internal Medicine, General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia

Department of Internal Medicine
Maja Šeruga, Department of Internal Medicine, General Hospital Murska Sobota, Murska Sobota, Slovenia

Department of Internal Medicine
Matej Završnik, Department of Endocrinology and Diabetology, Division of Internal Medicine, University Medical Centre Maribor, Maribor, Slovenia

Department of Endocrinology and Diabetology

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