Combination effect of cytochrome P450 1A1 gene polymorphisms on uterine leiomyoma: A case-control study

  • Saeedeh Salimi Cellular and Molecular Research Center and Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, Zahedan, Iran
  • Mojtaba Sajadian Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  • Maryam Khodamian Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan
  • Atefeh Yazdi School of Medicine, Zahedan University of Medical Sciences, Zahedan
  • Soodabeh Rezaee School of Medicine, Zahedan University of Medical Sciences, Zahedan
  • Abbas Mohammadpour-Gharehbagh Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan
  • Mojgan Mokhtari School of Medicine, Zahedan University of Medical Sciences, Zahedan; Zahedan University of Medical Sciences, Zahedan
  • Minoo Yaghmaie School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran
Keywords: Cytochrome P450 1A1, haplotype, gene, polymorphism, uterine leiomyoma

Abstract

Uterine leiomyoma (UL) is an estrogen-dependent neoplasm of the uterus, and estrogen metabolizing enzymes affect its progression. This study aimed to evaluate the association between two single-nucleotide polymorphisms of cytochrome P450 1A1 (CYP1A1) gene and UL risk. The study consisted of 105 patients with UL and 112 healthy women as controls. Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene were analyzed by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods, respectively. The findings indicated no association between Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene and UL (p < 0.05). However, the combination effect of TT/AG genotypes of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms was associated with 4.3-fold higher risk of UL. In addition, haplotype analysis revealed that TG haplotype of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms could increase the UL risk nearly 4.9-fold. Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms of CYP1A1 gene were not associated with UL susceptibility; however, the combination of the TT/AG genotypes and TG haplotype could increase the UL risk.

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Author Biographies

Saeedeh Salimi, Cellular and Molecular Research Center and Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, Zahedan, Iran

Cellular and Molecular Research Center; Department of Clinical Biochemistry

 

Mojtaba Sajadian, Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Cellular and Molecular Research Center; Department of Clinical Biochemistry

Maryam Khodamian, Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan

Cellular and Molecular Research Center; Department of Clinical Biochemistry

Atefeh Yazdi, School of Medicine, Zahedan University of Medical Sciences, Zahedan
Department of Obstetrics and Gynecology
Soodabeh Rezaee, School of Medicine, Zahedan University of Medical Sciences, Zahedan
Department of Obstetrics and Gynecology
Abbas Mohammadpour-Gharehbagh, Zahedan University of Medical Sciences, Zahedan; School of Medicine, Zahedan University of Medical Sciences, Zahedan

Cellular and Molecular Research Center; Department of Clinical Biochemistry

 

Mojgan Mokhtari, School of Medicine, Zahedan University of Medical Sciences, Zahedan; Zahedan University of Medical Sciences, Zahedan

Department of Obstetrics and Gynecology; Pregnancy Health Research Center

Minoo Yaghmaie, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran
Department of Obstetrics and Gynecology

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Published
2016-08-02
How to Cite
1.
Salimi S, Sajadian M, Khodamian M, Yazdi A, Rezaee S, Mohammadpour-Gharehbagh A, Mokhtari M, Yaghmaie M. Combination effect of cytochrome P450 1A1 gene polymorphisms on uterine leiomyoma: A case-control study. Bosn J of Basic Med Sci [Internet]. 2016Aug.2 [cited 2019Nov.13];16(3):209-14. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/1245
Section
Molecular Biology