ERBB4 as a therapeutic target in aortic dissection: Implications for cell-based therapies in vascular regeneration

Authors

  • Yu Shi Continuing Education College, Anhui Medical University, Hefei, Anhui, China; Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Jinjin Meng Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Shengqiang Zhang Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Shaofeng Yang Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Ge Liu Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Zhen Wu Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Chongwen Shen Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China
  • Chao Shi Continuing Education College, Anhui Medical University, Hefei, Anhui, China; Department of Cardiovascular surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China

DOI:

https://doi.org/10.17305/bb.2025.11925

Keywords:

Aortic dissection, AD, Erb-B2 receptor tyrosine kinase 4, ERBB4, vascular smooth muscle cells, VSMCs, cell-based therapy, NF-κB pathway

Abstract

This study investigates the role of Erb-B2 receptor tyrosine kinase 4 (ERBB4) in aortic dissection (AD) pathogenesis and its potential as a therapeutic target in cell-based therapies. Using immunohistochemical (IHC) staining, we examined the phenotypic transition of vascular smooth muscle cells (VSMCs) in thoracic aortic tissue from AD patients. RNA sequencing identified differentially expressed genes (DEGs), highlighting ERBB4 as a key regulator. In vitro, ERBB4 knockdown in human aortic smooth muscle cells (HASMCs) was assessed using Cell Counting Kit-8 (CCK-8 assays), wound healing, transwell migration, colony formation, flow cytometry, and tube formation assays. Our results showed significant inhibition of cell viability, migration, proliferation, and angiogenesis. In vivo, ERBB4 knockdown reduced inflammatory cell infiltration and enhanced collagen fiber contractility, as demonstrated by Masson staining. Mechanistically, ERBB4 silencing suppressed integrin-binding proteins (CD151, ITGAE, ITGB5) and inhibited NF-κB signaling (p-IκBα, p-NF-κB-65). These findings suggest that ERBB4 is critical in AD progression and that its knockdown mitigates pathological changes in HASMCs, reduces inflammation, and restores collagen contractility. ERBB4 may serve as a promising target for cell-based therapies aimed at restoring vascular integrity and function, providing potential new treatment strategies for AD patients.

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ERBB4 as a therapeutic target in aortic dissection: Implications for cell-based therapies in vascular regeneration

Published

14-03-2025

Issue

Section

Thematic issue: Cell-Based Therapies

How to Cite

1.
ERBB4 as a therapeutic target in aortic dissection: Implications for cell-based therapies in vascular regeneration. Biomol Biomed [Internet]. 2025 Mar. 14 [cited 2025 Mar. 30];. Available from: https://www.bjbms.org/ojs/index.php/bjbms/article/view/11925