Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study

  • Manal S. Fawzy Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia https://orcid.org/0000-0003-1252-8403
  • Ahmed A. Abdelghany Department of Ophthalmology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
  • Eman A. Toraih Department of Surgery, School of Medicine, Tulane University, New Orleans, Louisiana, USA; Department of Histology and Cell Biology (Genetics Unit), Faculty of Medicine, Suez Canal University, Ismailia, Egypt https://orcid.org/0000-0001-9267-3787
  • Abeer M. Mohamed Department of Clinical Pathology and Clinical Chemistry, Faculty of Medicine, Sohag University, Sohag, Egypt; Department of Clinical Laboratory Sciences, Al-Ghad International College for Applied Medical Sciences, Abha, Saudi Arabia https://orcid.org/0000-0002-3964-0289
Keywords: Long noncoding RNA, H19, GAS5, diabetic retinopathy, aflibercept, type 2 diabetes mellitus, T2DM

Abstract

Recently, a wide range of biological and pathological roles of long noncoding RNAs (lncRNAs) have been discovered. However, the potential role of circulating lncRNAs H19 and GAS5 in type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) is not clear. Here, we assessed the plasma levels of H19 and GAS5 lncRNAs in T2DM patients with/without DR and evaluated if H19 and GAS5 pre-treatment plasma levels are a predictor of early response to a single aflibercept dose in DR subgroup. Plasma lncRNA expression profiles of 119 T2DM patients (66 with DR and 53 without DR) and 110 healthy controls were determined by quantitative reverse transcription PCR. The association of lncRNA expression profiles with clinical features and aflibercept early response in DR patients was investigated. Relative H19 expression levels were significantly increased in T2DM group (including DR and non-DR subgroups) vs. controls, while GAS5 levels were decreased in T2DM group (p < 0.001). There was no significant difference in H19 and GAS5 expression levels between DR and non-DR subgroups. H19 and GAS5 expression profiles were not significantly correlated with clinical parameters or response to aflibercept therapy in DR subgroup. Our findings indicate that the circulating lncRNAs H19 and GAS5 may be associated with T2DM prevalence but may not have an important diagnostic/prognostic role in DR or early response to aflibercept intravitreal injection in DR patients. Large-scale transcriptomic studies are warranted to validate our results and investigate other lncRNA candidates in T2DM.

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Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study
Published
2020-08-03
How to Cite
1.
Fawzy MS, Abdelghany AA, Toraih EA, Mohamed AM. Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study. Bosn J of Basic Med Sci [Internet]. 2020Aug.3 [cited 2020Jul.12];20(3):365-71. Available from: http://bjbms.org/ojs/index.php/bjbms/article/view/4533
Section
Molecular Biology