A preliminary study of microRNA expression in different types of primary melanoma

MiRNA expression in primary melanoma

  • Ioana Gencia Department of Dermatology, "Victor Babeş" University of Medicine and Pharmacy; University Clinic of Dermatology and Venereology, Municipal Clinical Emergency Hospital of Timisoara, Timisoara, Romania https://orcid.org/0000-0002-2917-8051
  • Flavia Baderca Department of Microscopic Morphology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania https://orcid.org/0000-0002-5755-179X
  • Stefania Avram Department of Dermatology, "Victor Babeş" University of Medicine and Pharmacy; University Clinic of Dermatology and Venereology, Municipal Clinical Emergency Hospital of Timisoara, Timisoara, Romania https://orcid.org/0000-0002-3784-902X
  • Armand Gogulescu Department of Balneology, Medical Rehabilitation and Rheumatology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania
  • Anca Marcu Department of Biochemistry and Pharmacology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania https://orcid.org/0000-0003-3875-8143
  • Edward Seclaman Department of Biochemistry and Pharmacology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania https://orcid.org/0000-0003-1613-1884
  • Catalin Marian Department of Biochemistry and Pharmacology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania https://orcid.org/0000-0002-7749-1384
  • Caius Solovan Department of Dermatology, "Victor Babeş" University of Medicine and Pharmacy; University Clinic of Dermatology and Venereology, Municipal Clinical Emergency Hospital of Timisoara, Timisoara, Romania https://orcid.org/0000-0002-3784-902X
Keywords: microRNA, primary melanoma, miScript miRNA PCR Array

Abstract

MicroRNAs (miRNAs) have been proven to regulate the development and progression of cancer through various mechanisms. The aim of the present study was to compare miRNA expression between primary melanomas from different sites. We analyzed the expression of 84 miRNAs in 27 primary melanoma and 5 nevus formalin-fixed paraffin-embedded (FFPE) samples using the Human Cancer PathwayFinder miScript miRNA PCR Array. The FFPE samples were obtained from the archives of the Municipal Clinical Emergency Hospital of Timisoara and included 10 cutaneous melanomas, 10 uveal melanomas, 7 mucosal melanomas, and 5 cutaneous nevi. Out of 84 miRNAs, 11 miRNAs showed altered expression in all types of melanoma compared with the nevi. Among these, miR-155-5p, miR-9-5p, miR-142-5p, miR-19a-3p, miR-134-5p, and miR-301a-3p were upregulated, while miR-205-5p, miR-203a-3p, miR-27b-3p, miR-218-5p, and miR-23b-3p were downregulated. The highest similarity in miRNA expression pattern was found between uveal and mucosal melanoma groups, i.e., 15 miRNAs had altered expression in both groups. Overall, we identified several miRNAs with significantly altered expression in primary melanomas, including those reported for the first time in this type of cancer. Among them, mir-9-5p, mir-203a-3p, mir-19a-3p, mir-27b-3p, and mir-218-5p showed altered expression in all three melanoma types vs. nevi. Further research should explore the potential of these miRNAs in melanoma.

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A preliminary study of microRNA expression in different types of primary melanoma
Published
2019-08-27
How to Cite
1.
Gencia I, Baderca F, Avram S, Gogulescu A, Marcu A, Seclaman E, Marian C, Solovan C. A preliminary study of microRNA expression in different types of primary melanoma. Bosn J of Basic Med Sci [Internet]. 2019Aug.27 [cited 2019Oct.22];00. Available from: http://bjbms.org/ojs/index.php/bjbms/article/view/4271
Section
Molecular Biology