In vitro activity of heather [Calluna vulgaris (L.) Hull] extracts on selected urinary tract pathogens

  • Dragana M Vučić University of Banja Luka
  • Miroslav R Petković University of Banja Luka
  • Branka B Rodić-Grabovac University of Banja Luka
  • Olgica D Stefanović University of Kragujevac
  • Sava M Vasić University of Kragujevac
  • Ljiljana R Čomić University of Kragujevac
Keywords: heather, phenols, flavonoids, antibacterial activity, drug activity


Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract.


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Author Biographies

Dragana M Vučić, University of Banja Luka
Medical Faculty, Department of Microbiology
Miroslav R Petković, University of Banja Luka
Medical Faculty, Department of Microbiology
Branka B Rodić-Grabovac, University of Banja Luka
Faculty of Tehnology, Department of Organic Chemistry
Olgica D Stefanović, University of Kragujevac
Faculty of Science, Department of Biology and Ecology
Sava M Vasić, University of Kragujevac
Faculty of Science, Department of Biology and Ecology
Ljiljana R Čomić, University of Kragujevac
Faculty of Science, Department of Biology and Ecology


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In vitro activity of heather [Calluna vulgaris (L.) Hull] extracts on selected urinary tract pathogens
How to Cite
Vučić DM, Petković MR, Rodić-GrabovacBB, Stefanović OD, Vasić SM, Čomić LR. In vitro activity of heather [Calluna vulgaris (L.) Hull] extracts on selected urinary tract pathogens. Bosn J of Basic Med Sci [Internet]. 2014Nov.15 [cited 2021Jan.27];14(4):234-8. Available from: