Effect of topically applied sildenafil citrate on wound healing: experimental study

  • Koray Gürsoy ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC ANKARA
  • Melike Oruç ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
  • Yüksel Kankaya ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
  • Mustafa Gürhan Ulusoy ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
  • Uğur Koçer ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC, ANKARA
  • Duygu Kankaya Ankara University, Pathology Department, ANKARA
  • Reyhan Neslihan Gürsoy Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, ANKARA
  • Özge Çevik Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, ANKARA, TURKEY
  • Elmas Öğüş Ankara Research and Training Hospital, Biochemistry Clinic, ANKARA, TURKEY
  • Vildan Fidanci Ankara Research and Training Hospital, Biochemistry Clinic, ANKARA, TURKEY
Keywords: Wound healing, Sildenafil Citrate, cGMP

Abstract

Wound healing is a complex process that necessitates organization of different cell types and several signalling molecules. The aim of this study is to evaluate the effect of different concentrations of sildenafil citrate, which decreases cGMP degradation, on wound healing by secondary intention.

This study was performed using 25 Sprague Dawley rats weighing 200-250 grams. 4 dorsal defects were created. Four different treatment modalities which were 1% and 5% sildenafil citrate gel prepared with carbopol, pure carbopol gel without any drug in it and 0,9% NaCl solution; were applied to each lesion of the same rat. Randomly selected five rats (25 rats in total) were sacrificed on 3rd, 5th, 7th, 10th, and 14th days; and the effect of each modality was evaluated by means of defect area measurement, histopathological examination and measurement of tissue hydroxyproline levels.

Sildenafil citrate gel application decreased the defect areas in a dose independent manner starting from 3rd day and dose dependent manner after 7th day. By means of vascularization, sildenafil citrate increased vascularity starting from 3rd day. The strength of acute inflammation was superior in sildenafil groups starting from 5th day; and the amount and maturation of granulation in the wound bed, as well as the strength of chronic inflammation were superior in defects treated with sildenafil citrate as early as 7th day.

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Effect of topically applied sildenafil citrate on wound healing: experimental study
Published
2014-08-16
How to Cite
1.
Gürsoy K, Oruç M, Kankaya Y, Ulusoy MG, Koçer U, Kankaya D, Gürsoy RN, Çevik Özge, Öğüş E, Fidanci V. Effect of topically applied sildenafil citrate on wound healing: experimental study. Bosn J of Basic Med Sci [Internet]. 2014Aug.16 [cited 2020Aug.8];14(3):125-31. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/125
Section
Biochemistry