Radiographic estimation in seropositive and seronegative rheumatoid arthritis

Long since it have been suggested that a subpopulation of patients with rheumatoid arthritis, diagnosed with negative rheumatoid factor tests, represents a clinical entity quite distinct from that of seropositive rheumatoid arthritis (RA). Our aim was to establish a scientifi c comparative analysis between seronegative and seropositive rheumatoid arthritis, regarding some radiological and clinical parameters, applied for the fi rst time on patients from Kosovo. Two hundred fi fty patients with rheumatoid arthritis according to the American College of Rheumatology criteria were retrospectively studied by analysis the radiographic damage and clinical parameters of the disease, using a data base. All examinees were between - years of age (Xb=., SD=.) with disease duration between - years (Xb = ., SD=.). All patients underwent a standardised evaluation radiographs. Baseline standardised poster anterior radiographs of hands and feet and radiographs of other joints, depending on indications, were assessed. Erythrocyte sedimentation rate values correlated with the radiological damages and statistical diff erence was found for seronegative subset (r=., p<.). Longer duration of the disease resulted in the increase of radiological changes in both subsets (r=., p<.) seronegative, (r=., p<.) seropositive. Anatomic changes of IInd and IIIrd level were nearly equally distributed in both subsets,  (.) seronegative,  () seropositive. Radiological damages are nearly equal in both subsets, elevate in relation to the duration of the disease and correlate with ESR values. Regarding the sero-status, diff erences within sex, with some exceptions, are not relevant. Although there are some defi nite quantitative and qualitative diff erences regarding sero-status, obviously there is a great deal of overlap between the two groups. ©  Association of Basic Medical Sciences of FBIH. All rights reserved


INTRODUCTION
Rheumatoid arthritis (RA) is an auto-immune, chronic inflammatory disease characterised by synovitis and bone destruction [].Although the etiopathogenesis of RA is unknown, the majority of scientists support the immunology based theory on discovery of rheumatoid factor (RF) [].A positive test for rheumatoid factor is by no means pathognomonic of rheumatoid arthritis, but is present in  to  of patients with the disease, as well as in - in healthy population.Patients with a high titer of IgM-RF are more likely to have erosive joint disease, extra-articular manifestations, and greater functional disability.In contrast, patients with negative rheumatoid factor in general exhibit a milder disease course.Recently, various test methods based on the principle of agglutination (Waaler-Rose and Latex tests) are being applied, by which only the presence of IgM-RF is proven.Rheumatoid factor could be found in diff erent immunoglobuline classes (G, A, D and E) defi ned by ELISA [].Th e infl ammation in RA causes a shift in the bone metabolism towards increased osteoclast -mediated bone turn-over [].Th is dysregulation causes reduced bone mass, which is known to be an early feature in RA patients, visualised as juxta-articular bone demineralisation on radiographs [].One of the  diagnostic criteria for the diagnoses of RA, established by the American College of Rheumatology (ACR) in , is the presence of bone erosion on radiograph [].Genetic information is necessary for prediction of radiographical changes in patients with RA.Severe radiological changes are associated with allele HLA-DRB*.Within  years of disease onset, approximately  of all patients develop erosive disease, and show a light progress from the ninth year onwards.Th e patients with erosion, particularly on feet, in the early phase of disease are associated with a destructive course of RA [].Th e same problem appears in patients with arthritis of large joints at fi rst presentation, in particular the knee [].Radiographic progression in rheumatoid arthritis has in several studies been shown to be predicted by serological markers widely used in daily clinical practice [, ].Quantifi cation of localised bone loss has been proposed as an outcome measure in early RA [].Plain X-ray off ers high specifi city in the differential diagnoses of rheumatic diseases [].Th ere are other useful tools like Magnetic Resonance Imaging (MRI), Computed Tomography (CT), Doppler Sonography, Bone Scintigraphy, Ultrasonography, etc., which are suitable for evaluating the intensity of synovitis, for early diagnosis of synovitis, and for the assessment of joints and periarticular structures in all rheumatological disorders respectively [,].In response to the continuing debate as to whether seronegative and seropositive rheumatoid arthritis are part of the same disease spectrum, or are distinct disorders, we aimed to perform a comparative analysis regarding some clinical and radiological features.

Patients
Using the data base,  patients with rheumatoid arthritis, diagnosed according to the American College of Rheumatology ACR () revised diagnostic criteria, were retrospectively studied by analysis the radiographic damage and clinical parameters of the disease, using the data base.The studied group consisted of  ( female,  male) seronegative patients with titers lower than / as defi ned by Rose-Waaler test, whereas the control group consisted of  ( female,  male) seropositive patients with titers of / or higher.Patients who belonged to  nd and  rd functional class (ARA) are taken into consideration.Th eir age ranged from  to  years (Xb=.)(seronegative Xb=.,SD=.,seropositive Xb=.,SD=.).Disease duration was between - years (Xb=.)(seronegative Xb=.,SD=., seropositive Xb=.,SD=.).At baseline, all patients underwent a standardized evaluation including laboratory tests and radiographs.Conventional hand radiographs were used as a "test subject".Patients belonged to II-IV anatomic stage (ARA).Erythrocyte sedimentation rate (ESR) was measured by the Westergren method, ranging from  to  mm/h.The correlation between diff erent clinical parameters, laboratory and anatomic stages were investigated.For the presentation of the results the structure, prevalence, arithmetic average (Xb), standard deviation (SD), variation coefficient (CV) and variation interval (Rmax-Rmin) were used.Probability level was expressed by p<. and p<..The correlation between the duration of RA and anatomical stages (ARA), ESR and anatomical stages (ARA), regarding sero-status was measured by Point-biserial correlation.

DISCUSSION
Patients with clinical features of rheumatoid arthritis, but negative rheumatoid factor present a diagnostic challenge.It has recently been suggested that a subpopulation of patients with RA, diagnosed on clinical, radiologic and pragmatic grounds, but with negative rheumatoid factor tests, represents a clinical entity quite distinct from that of seropositive RA [].Th e nature of the destructive process, as defined by radiological examination, may be different in patients with seropositive rheumatoid arthritis from that seen in individuals with so-called 'seronegative rheumatoid arthritis' [].Presence of anti-cyclic citrullinated peptide antibodies is correlated to disease activity and to bone erosions development [].Vittecoq et al. [] have concluded that the antibody anti-CCP, compared with rheumatoid factor, is of insuffi cient value to predict an early erosive and progressive RA, while Vencovsky [] considers that combined analysis of above parameters increase this possibility.
Th is study was undertaken to determine whether the two populations diff er radiologically.It was found that the diff erences between sero-groups in relation to anatomical stages did not show signifi cant statistical diff erence, which is valid to both sexes.Opinions of some authors [] who confi rm that seropositivity did not correlate with bone erosion are close to these fi ndings.Unlike our data, some authors claim that seropositivity results in more severe anatomical changes in joints [, , ] and those patients with high titer of IgM-RF have signifi cantly higher progressive radiological index [].Related data provided by el-Khoury et al.
[] con- fi rmed that radiograms of seronegative patients diff er significantly from radiograms of seropositive patients concerning the lower rate of juxta-articular osteoporosis, relative lack of subchondral erosion, predominance of changes across the carpal part, greater number of contractures and the asymmetry of the attacked joint.To this perception contributes the study of Krahe et al. [] as well, confi rming that the extent of periarticular destruction was signifi cantly greater amongst seropositive than amongst seronegative patients, both at the beginning and the end of the study, but there was no significant diff erence in the rate at which this progressed.HLA-DR alleles such as HLA-DR and HLA-DR are associated with the risk to develop RA [].Listing et al. [] have noticed no signifi cant diff erences in frequency of DR, in the patients with and without erosion, while Vehe et al. [] and Reneses et al. [] have found that DR is prevalent in the patients with erosive RA, regardless of the status of FR.Furthermore, in patients with RA, Mattey et al. [] have found that DRB are not predictive for erosive damages to early seropositive patients, but are predictive for seronegative patients.Anatomic damages are proportional to the intensity and the duration of the infl ammatory process, deteriorate rapidly during the fi rst  years of the disease, show a weak progress from the th year of duration and onward [].Much of these findings were confirmed in our study, where seropositive patients pass earlier in anatomic stages III-IV and that the duration of the disease increases radiological changes, with no statistically signifi cant diff erence according to sero-status.Currently available biomarkers of more severe disease include elevated ESR or C-reactive protein levels (CRP) and IgM-RF or the antibody anti-CCP positivity [,].Erythrocyte sedimentation rate is more closely related to the progression of joint damage than C-reactive protein or hemoglobin [].Findings in some studies add to the understanding of the antibody anti-CCP and ESR as important predictors of bone involvement in RA [].Our fi ndings provide evidence that accelerated ESR values correlate with anatomical stages (r=.,p<.).Greater correlation and positive (r=.,p<.) was found among seronegative patients, whereas seropositive group correlation was smaller and not of any statistical signifi cance (r=.,p>.).Our collected data are comparable to those of van Leeuwen et al. [] who confi rm that acute phase proteins are in correlation with radiological damages.Some authors consider that erosive damages at st year in patients with recent-onset RA are significantly influenced by SE homozygosity and the presence of baseline erosions, but not by RF status, anti-CCP status, or- TNF-alpha genotype [] the others claim that female gender, DRB* alleles (rather than the SE), and the presence of anti-CCP antibodies at baseline (independently of the titer) are the most important predictors of progression [].

CONCLUSION
Radiological damages are nearly equal in both subsets, elevate in relation to the duration of the disease and correlate with ESR values.Regarding the sero-status, differences within sex, with some exceptions, are not relevant.Although there are some definite quantitative and qualitative differences regarding sero-status, obviously there is a great deal of overlap between the two groups.

DECLARATION OF INTEREST
Authors declare no confl ict of interest.

TABLE 3 .
Correlation between accelerated values of ESR and anatomical stages (ARA) according to sero-status

TABLE 1 .
Radiological changes (ARA) regarding to sero-status and sex

TABLE 2 .
Correlation between the duration of AR and anatomical stages (ARA) according to sero-status tive].Diff erences between sero-groups, regarding anatomical classes were not signifi cant (χ  =., p>.).Apart from this, no signifi cant statistical diff erence regarding sex was found.,p<.).Greater correlation and positive (r=.,p<.) was found among seronegative patients, while seropositive group correlation was smaller and of no statistical significance (r=.,p>.)(Figure,).