Ukrain (NSC 631570) ameliorates intestinal ischemia-reperfusion-induced acute lung injury by reducing oxidative stress

Authors

  • Cengiz Kocak Department of Pathology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey http://orcid.org/0000-0003-3819-6822
  • Fatma Emel Kocak Department of Medical Biochemistry, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey http://orcid.org/0000-0001-5549-3883
  • Raziye Akcilar Department of Physiology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Aydin Akcilar Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Bircan Savran Department of Pediatric Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Sezgin Zeren Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Zulfu Bayhan Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Zeynep Bayat Department of Biochemistry, Faculty of Art and Science, Dumlupinar University, Kutahya, Turkey

DOI:

https://doi.org/10.17305/bjbms.2016.875

Keywords:

Ischemia-reperfusion injury, lung injury, oxidative stress, ukrain

Abstract

Intestinal ischemia-reperfusion (I/R) causes severe destruction in remote organs. Lung damage is a frequently seen complication after intestinal I/R. Ukrain (NSC 631570) is a synthetic thiophosphate derivative of alkaloids from the extract of the celandine (Chelidonium majus L.) plant. We investigated the effect of Ukrain in animals with lung injury induced by intestinal I/R. Adult male Spraque-Dawley rats were randomly divided into four groups: control, Ukrain, I/R, I/R with Ukrain. Before intestinal I/R was induced, Ukrain was administered intraperitoneally at a dose of 7.0 mg/body weight. After 1 h ischemia and 2 h reperfusion period, lung tissues were excised. Tissue levels of total oxidative status (TOS), total antioxidant status (TAS) were measured and oxidative stress indices (OSI) were calculated. Lung tissues were also examined histopathologically. TOS and OSI levels markedly increased and TAS levels decreased in the I/R group compared to the control group (P < 0.05). TOS and OSI levels markedly decreased and TAS levels increased in the I/R with Ukrain group compared with the group subjected to IR only (P < 0.05). Severe hemorrhage, alveolar septal thickening, and leukocyte infiltration were observed  in the I/R group. In the I/R with Ukrain group, morphologic changes occurring as a result of lung damage attenuated and histopathological scores reduced compared to the I/R group (P < 0.05). Our results suggest that Ukrain pretreatment could reduce lung injury induced by intestinal I/R induced via anti-inflammatory and antioxidant effects. 

Author Biographies

  • Cengiz Kocak, Department of Pathology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
    Department of Pathology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Fatma Emel Kocak, Department of Medical Biochemistry, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Department of Medical Biochemistry, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

  • Raziye Akcilar, Department of Physiology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
    Department of Physiology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey
  • Aydin Akcilar, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

  • Bircan Savran, Department of Pediatric Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Department of Pediatric Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

  • Sezgin Zeren, Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

  • Zulfu Bayhan, Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

  • Zeynep Bayat, Department of Biochemistry, Faculty of Art and Science, Dumlupinar University, Kutahya, Turkey

    Department of Biochemistry, Faculty of Art and Science, Dumlupinar University, Kutahya, Turkey

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Ukrain (NSC 631570) ameliorates intestinal ischemia-reperfusion-induced acute lung injury by reducing oxidative stress

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15-01-2016

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1.
Ukrain (NSC 631570) ameliorates intestinal ischemia-reperfusion-induced acute lung injury by reducing oxidative stress. Biomol Biomed [Internet]. 2016 Jan. 15 [cited 2024 Mar. 28];16(1):75-81. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/875