Possible association of ABCB1:c.3435T>C polymorphism with high-density-lipoprotein-cholesterol response to statin treatment - a pilot study.

  • Anna Sałacka Department of Family Medicine, Pomeranian Medical University, Szczecin, Poland
  • Agnieszka Bińczak-Kuleta Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland
  • Mariusz Kaczmarczyk Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland
  • Iwona Hornowska Department of Family Medicine, Pomeranian Medical University, Szczecin, Poland
  • Krzysztof Safranow Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
  • Jeremy Simon Cabot Clark Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Szczecin, Poland
Keywords: ABCB1 transporter, HMG-CoA reductase inhibitors, P-glycoprotein

Abstract

The gene product ABCB1 (formerly MDR1 or P-glycoprotein) is hypothesized to be involved in cholesterol cellular trafficking, redistribution and intestinal re-absorption. Carriers of the ABCB1:3435T allele have previously been associated with decreases in ABCB1 mRNA and protein concentrations and have been correlated with changes in serum lipid concentrations. The aim of this study was to investigate possible association between the ABCB1:3435T>C polymorphism and changes in lipids in patients following statin treatment. Outpatients (n=130) were examined: 43 men (33%), 87 women (67%): treated with atorvastatin or simvastatin (all patients with equivalent dose of 20 or 40 mg/d simvastatin). Blood was taken for ABCB1:3435T>C genotyping, and before and after statin treatment for lipid concentration determination (total cholesterol, high-density-lipoprotein-cholesterol (HDL-C), triglycerides). Change (Δ) in lipid parameters, calculated as differences between measurements before and after treatment, were analyzed with multiple regression adjustments: gender, diabetes, age, body mass index, equivalent statin dose, length of treatment. Univariate and multivariate analyses showed significant differences in ΔHDL-C (univariate p=0.029; multivariate p=0.036) and %ΔHDL-C (univariate p=0.021; multivariate p=0.023) between patients with TT (-0.05 ± 0.13 g/l; -6.8% ± 20%; respectively) and CC+CT genotypes (0.004 ± 0.15 g/l; 4.1 ± 26%; respectively). Reduction of HDL-C in homozygous ABCB1:3435TT patients suggests this genotype could be associated with a reduction in the benefits of statin treatment.

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Published
2014-08-14
How to Cite
1.
Sałacka A, Bińczak-Kuleta A, Kaczmarczyk M, Hornowska I, Safranow K, Clark JSC. Possible association of ABCB1:c.3435T>C polymorphism with high-density-lipoprotein-cholesterol response to statin treatment - a pilot study. Bosn J of Basic Med Sci [Internet]. 2014Aug.14 [cited 2019Dec.9];14(3):144-9. Available from: http://bjbms.org/ojs/index.php/bjbms/article/view/144
Section
Molecular Biology